What machines do pathologists use?

July 25, 2022

Pathology has made significant advances thanks to the use of genomic-based molecular techniques such as polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), array comparative genomic hybridization (ACGH), and, more recently, DNA sequencing.

Basic Supplies:

1. microtome

2. Biomedical Specimen Storage Cabinet

3. Automatic Slide Stainer

4. cooling plate

5. A trimmer or dispenser for paraffin

6. Slide Dryer

7. Tissue embedding system

8. floating tissue system

1. microtome :

A microtome (from the Greek Mikros, that means "small," and temnein, which means "to cut") is a cutting tool that produces extremely thin slices of material known as sections. Microtomes are important in science because those that allow for the preparation of samples for observation under transmitted light or electron radiation.

Depending on the specimen being sliced and the desired thickness of the sections being cut, microtomes use steel, glass, or diamond blades. To prepare histological sections of animal or plant tissues for light microscopy, steel blades are used. Glass knives are used to cut light microscopy sections as well as very thin sections for electron microscopy. Diamond knives of industrial grade are used to slice hard materials such as bone, teeth, and tough plant matter for light and electron microscopy. Diamond knives of the highest quality are also used to cut thin sections for electron microscopy.

2. Biomedical Specimen Storage Cabinet:

As the down flow, the Class II Type B1 biological safety cabinet has a common plenum from which 70% of the air is exhausted and 30% is re-circulated to the work area. Type B1 cabinets also have a dedicated exhaust

feature that eliminates re-circulation when working towards the back of the cabinet's interior. Toxic chemicals should only be used as an adjunct to microbiological processes if they do not interfere with work when re-circulated in the down flow.

3. Automatic Slide Stainer:

The ATS200 Automatic Slide Stainer is a pathology and research lab workstation automatic slides stainer. ATS 200 is designed for staining applications in pathology and research. It can perform multiple staining protocols simultaneously and allows for continuous unloading and reloading without interrupting the staining cycle.

4. cooling plate:

Cold plates are typically made of metal and have flow paths machined into them to allow a heat-transfer fluid to pass through. As the plates absorb electrical waste heat, they dissipate it via liquid cooling through the flow paths.

5. A trimmer or dispenser for paraffin:

A paraffin or wax dispenser melts the solid paraffin and keeps it molten so that it can be dispensed as needed. The Medimeas Paraffin

Dispenser is CE certified and comes in 5L and 10L capacities.

6. Slide Dryer:

The dryer is designed to rapidly dry slides by blowing warm air across their surface. The slide dryer accepts two slide racks directly from a stainer and allows the slides to be stained in 20-30 minutes.

7. Tissue embedding system:

Embedding is the process of surrounding tissues with a medium such as paraffin wax. Tissue orientation in melted paraffin that, when solidified, provides adequate external support during sectioning by keeping all parts of the tissue intact when sections are cut.

8.floating tissue system:

Aerenchyma is the tissue that allows aquatic plants to float. Aerenchyma is essentially parenchyma tissue in aquatic plants. Aerenchyma cells form a network with large air spaces or air cavities for gaseous exchange.

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Reference Digital Pathology UCGconferences press releases and blogs

https://medium.com/@taania.ucg/what-is-digital-pathology-932897b40e03

https://kikoxp.com/posts/13185

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https://sites.google.com/view/digitalpathologyucg/what-is-digital-pathology

https://digitalpathologyucg.blogspot.com/2022/07/what-is-digital-pathology.html

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https://www.tumblr.com/dashboard

https://medium.com/@taania.ucg/there-are-different-types-of-microtomes-554ba2510e9d