There are different types of microtomes

 

There are different types of microtomes :      

1.     1. hand microtome

2.     2.  rotational microtome

3.     3. sledge microtome

4.    4. cryostat (freezing microtome)

5.   5.  ultramicrotome

11. hand microtome:

Hand Thin sections of microscopic materials, such as specimen parts of plants and animals, can be cut with a microtome. It is regarded as the fundamental design of a microtome, allowing quick and precise manual microtomic cuts. The microtome is appropriate for fieldwork during excursions, school instruction, practical microscopy classes, and biology enthusiasts who take their hobby seriously.

2. rotational microtome:

A manually operated cutting tool for all types of paraffin and hard cutting tasks in the fields of biology, medicine, and industry is the microTec rotary microtome. Making microscopic preparations for a subsequent illumination is done using it (e.g., human tissue)

3 .sledge microtome:

The sample is put into a fixed holder (shuttle) on a sledge microtome, which then moves back and forth across a knife. Modern sled microtomes have a linear bearing on which the sled is mounted; this design enables the microtome to quickly cut several coarse pieces. The pressure put on the sample during the cut can be decreased by altering the angles between the sample and the microtome knife. The preparation of big samples, like those encased in paraffin for biological preparations, is a typical application for this type of microtome. A sledge microtome can typically achieve cut thicknesses of 1 to 60 m.

44. cryostat (freezing microtome)

The cryostat is essentially a microtome, an ultrafine "deli-slicer," placed in a freezer. A stationary upright freezer with an external wheel to rotate the microtome is typically used as a cryostat. Depending on the tissue being cut, the temperature might range from minus 20 to negative 30 degrees Celsius.

5. ultramicrotome.

Using a transmission electron microscope, ultramicrotomy is a technique for slicing objects into incredibly thin slices, or ultra-thin sections, that may be examined and recorded at various magnifications (TEM). Although sections of plastic and soft metals can also be made, biological specimens are the majority of what are used. Sections must be extremely thin since biological material thicker than 150 nm cannot be seen using the typical electron microscope's 50 to 125 kV electrons. Sections between 30 and 60 nm should be used for the best resolution. This is about similar to cutting a single red blood cell into 100 slices, or slicing a 0.1 mm thick human hair into 2,000 slices along its diameter.

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